Part:BBa_I761013:Design

Insulin A generator with TAT signal peptide with glucose-inducible promoter

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

No double terminator in the end of this construct DNA of insulin Alpha chain has a stop codon in the end

Source

glucose-inducible promoter is derived from biobrick BBa_R0082 insulin Alpha chain is derived from MGC clone (NCBI accession number: BC005255) TAT signal peptide is derived from E. coli K12 genomic DNA